Composite

Part:BBa_K1025003:Design

Designed by: Weifan Liang   Group: iGEM13_Tsinghua-E   (2013-09-14)


Thu-E Mutation Part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 478
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1770
    Illegal AgeI site found at 73
    Illegal AgeI site found at 350
    Illegal AgeI site found at 839
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 55
    Illegal SapI.rc site found at 1061


Design Notes

The genes were cloned easily by PCR and connected to plasmid backbone by in-fusion cloning.


Source

MutD comes from E.coli W3110 genome with two animo acid mutation[1]. GFP was conserved in our lab and its GenBank accession number is KF020493.1. [1]Schaaper, R. M. MECHANISMS OF MUTAGENESIS IN THE ESCHERICHIA-COLI MUTATOR MUTD5 - ROLE OF DNA MISMATCH REPAIR. Proc. Natl. Acad. Sci. U. S. A.85, 8126-8130, doi:10.1073/pnas.85.21.8126 (1988)

References